IFI-A · Tea-Black Fermentation

Specimen SS-F-AST-856 // ASTRINGENCY · IFI-A

Tea-Black Fermentation II-IV-B

Camellia sinensis

Tea

Tier IITea-Black Fermentation

Target Trait

2.24×

fold-change

Theaflavin/thearubigin equivalent oxidative coupling index

Technical Specifications

SKU

SS-F-AST-856

Genomic Edits

5

GC Content

47.3%

Construct Length

3,114 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

224

Pathway Edits

  • PPO1 overexpression (polyphenol oxidase 1) for catechin ortho-quinone formation and theaflavin initiation
  • LAC3 laccase amplification for complementary oxidative coupling of flavan-3-ol substrates
  • CYP78A5 upregulation for subsidiary oxidative modification of flavan-3-ol substrates
  • peroxidase-1 co-expression for H2O2-dependent oxidative coupling in thearubigin formation
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PPO1-driven catechin ortho-quinone formation for theaflavin benzotropolone ring condensationLAC3/peroxidase-1 complementary oxidative coupling for thearubigin analog formationCYP78A5 auxiliary oxidative modification for expanded oxidative astringency architectureSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
224
IFI-F
—

Use Case

Craft black tea analog ingredient development, specialty oxidative astringency ingredient sourcing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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