IFI-A · Tea-Black Fermentation

Specimen SS-F-AST-847 // ASTRINGENCY · IFI-A

Tea-Black Fermentation I-IV-A

Camellia sinensis

Tea

Tier ITea-Black Fermentation

Target Trait

1.4×

fold-change

Theaflavin/thearubigin equivalent oxidative coupling index

Technical Specifications

SKU

SS-F-AST-847

Genomic Edits

3

GC Content

44.7%

Construct Length

2,370 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

140

Pathway Edits

  • PPO1 overexpression (polyphenol oxidase 1) for catechin ortho-quinone formation and theaflavin initiation
  • LAC3 laccase amplification for complementary oxidative coupling of flavan-3-ol substrates
  • CYP78A5 upregulation for subsidiary oxidative modification of flavan-3-ol substrates
  • peroxidase-1 co-expression for H2O2-dependent oxidative coupling in thearubigin formation
  • Cell-free wheat germ extract reconstitution system

Key Interventions

PPO1-driven catechin ortho-quinone formation for theaflavin benzotropolone ring condensationLAC3/peroxidase-1 complementary oxidative coupling for thearubigin analog formationCYP78A5 auxiliary oxidative modification for expanded oxidative astringency architectureSP6/T7 dual-polymerase cell-free transcription/translation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
140
IFI-F
—

Use Case

Black tea oxidation pathway research, entry-level theaflavin engineering, academic tea fermentation oxidative chemistry.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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