Specimen SS-F-AST-832 // ASTRINGENCY · IFI-A
Wine-Style Tannin Architecture IV-IV-B
Vitis vinifera
Grape
Target Trait
4.17×
fold-change
Bordeaux-style epicatechin:catechin proanthocyanidin ratio
Technical Specifications
SKU
SS-F-AST-832
Genomic Edits
9
GC Content
46.2%
Construct Length
4,688 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
417
Pathway Edits
- LAR balanced expression (leucoanthocyanidin reductase) for catechin starter unit control
- ANR high-flux variant (anthocyanidin reductase) for epicatechin extension unit dominance
- DFR-EC epicatechin-favoring dihydroflavonol 4-reductase allele overexpression
- F3H substrate channeling (flavanone 3-hydroxylase) for dihydroquercetin precursor flux
- TT12 vacuolar transport for procyanidin structure-preserving sequestration
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Industrial Bordeaux-style tannin ingredient production, premium wine style engineering, beverage processing R&D. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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