IFI-A · Wine-Style Tannin Architecture

Specimen SS-F-AST-814 // ASTRINGENCY · IFI-A

Wine-Style Tannin Architecture II-PR-B

Vitis vinifera

Grape

Tier IIWine-Style Tannin Architecture

Target Trait

2.61×

fold-change

Bordeaux-style epicatechin:catechin proanthocyanidin ratio

Technical Specifications

SKU

SS-F-AST-814

Genomic Edits

6

GC Content

46.8%

Construct Length

3,580 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

261

Pathway Edits

  • LAR balanced expression (leucoanthocyanidin reductase) for catechin starter unit control
  • ANR high-flux variant (anthocyanidin reductase) for epicatechin extension unit dominance
  • DFR-EC epicatechin-favoring dihydroflavonol 4-reductase allele overexpression
  • F3H substrate channeling (flavanone 3-hydroxylase) for dihydroquercetin precursor flux
  • TT12 vacuolar transport for procyanidin structure-preserving sequestration
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

LAR/ANR differential tuning for Bordeaux-style epicatechin:catechin dominanceDFR-EC allelic selection for epicatechin precursor substrate preferenceTT12 transport for vacuolar sequestration of Bordeaux-style procyanidin architecturePEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
261
IFI-F
—

Use Case

Craft Bordeaux-style wine analog development, specialty wine tannin ingredient sourcing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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