IFI-A · Wine-Style Tannin Architecture

Specimen SS-F-AST-807 // ASTRINGENCY · IFI-A

Wine-Style Tannin Architecture I-IV-A

Vitis vinifera

Grape

Tier IWine-Style Tannin Architecture

Target Trait

1.6×

fold-change

Bordeaux-style epicatechin:catechin proanthocyanidin ratio

Technical Specifications

SKU

SS-F-AST-807

Genomic Edits

4

GC Content

44.7%

Construct Length

2,870 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

160

Pathway Edits

  • LAR balanced expression (leucoanthocyanidin reductase) for catechin starter unit control
  • ANR high-flux variant (anthocyanidin reductase) for epicatechin extension unit dominance
  • DFR-EC epicatechin-favoring dihydroflavonol 4-reductase allele overexpression
  • F3H substrate channeling (flavanone 3-hydroxylase) for dihydroquercetin precursor flux
  • TT12 vacuolar transport for procyanidin structure-preserving sequestration
  • Cell-free wheat germ extract reconstitution system

Key Interventions

LAR/ANR differential tuning for Bordeaux-style epicatechin:catechin dominanceDFR-EC allelic selection for epicatechin precursor substrate preferenceTT12 transport for vacuolar sequestration of Bordeaux-style procyanidin architectureSP6/T7 dual-polymerase cell-free transcription/translation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
160
IFI-F
—

Use Case

Wine style tannin research, entry-level Bordeaux profile engineering, academic epicatechin:catechin ratio studies.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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