Wine-Style Tannin Architecture I-PR-B

Vitis vinifera

Grape

Tier IWine-Style Tannin Architecture

Target Trait

1.84×

fold-change

Bordeaux-style epicatechin:catechin proanthocyanidin ratio

Technical Specifications

SKU

SS-F-AST-806

Genomic Edits

GC Content

48.5%

Construct Length

3,233 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

184

Pathway Edits

LAR balanced expression (leucoanthocyanidin reductase) for catechin starter unit control
ANR high-flux variant (anthocyanidin reductase) for epicatechin extension unit dominance
DFR-EC epicatechin-favoring dihydroflavonol 4-reductase allele overexpression
F3H substrate channeling (flavanone 3-hydroxylase) for dihydroquercetin precursor flux
TT12 vacuolar transport for procyanidin structure-preserving sequestration
Protoplast PEG-fusion transient and stable integration protocol
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

LAR/ANR differential tuning for Bordeaux-style epicatechin:catechin dominanceDFR-EC allelic selection for epicatechin precursor substrate preferenceTT12 transport for vacuolar sequestration of Bordeaux-style procyanidin architecturePEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

184

IFI-F

—

Use Case

Wine style tannin research, entry-level Bordeaux profile engineering, academic epicatechin:catechin ratio studies. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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