Raspberry Ellagitannin V-PR-B

Rubus idaeus

Raspberry

Tier VRaspberry Ellagitannin

Target Trait

4.37×

fold-change

Sanguiin H-6/lambertianin C ellagitannin accumulation

Technical Specifications

SKU

SS-F-AST-798

Genomic Edits

GC Content

46.9%

Construct Length

4,741 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

437

Pathway Edits

UGGT overexpression (galloyl-UDP-glucosyltransferase) for galloyl-glucose platform establishment in Rubus
laccase-RAG amplification (Rubus-associated galloyl oxidase) for C-C oxidative coupling in sanguiin H-6 formation
BAHD-GalT galloyltransferase upregulation for sequential galloylation of glucose scaffold
PGT polygalloylglucoside transferase co-expression for lambertianin C extension
Protoplast PEG-fusion transient and stable integration protocol
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

UGGT/laccase-RAG coupled galloyl-glucose ester formation and oxidative C-C couplingBAHD-GalT sequential galloylation for sanguiin H-6 scaffold assemblyPGT polygalloylation for lambertianin C type ellagitannin polymerizationPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

437

IFI-F

—

Use Case

Flagship premium raspberry ellagitannin ingredient production, pharmaceutical-grade sanguiin H-6 standardization, ultra-premium functional berry extract manufacturing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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