IFI-A · Raspberry Ellagitannin

Specimen SS-F-AST-792 // ASTRINGENCY · IFI-A

Raspberry Ellagitannin IV-IV-B

Rubus idaeus

Raspberry

Tier IVRaspberry Ellagitannin

Target Trait

3.68×

fold-change

Sanguiin H-6/lambertianin C ellagitannin accumulation

Technical Specifications

SKU

SS-F-AST-792

Genomic Edits

8

GC Content

46.2%

Construct Length

4,428 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

368

Pathway Edits

  • UGGT overexpression (galloyl-UDP-glucosyltransferase) for galloyl-glucose platform establishment in Rubus
  • laccase-RAG amplification (Rubus-associated galloyl oxidase) for C-C oxidative coupling in sanguiin H-6 formation
  • BAHD-GalT galloyltransferase upregulation for sequential galloylation of glucose scaffold
  • PGT polygalloylglucoside transferase co-expression for lambertianin C extension
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

UGGT/laccase-RAG coupled galloyl-glucose ester formation and oxidative C-C couplingBAHD-GalT sequential galloylation for sanguiin H-6 scaffold assemblyPGT polygalloylation for lambertianin C type ellagitannin polymerizationSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
368
IFI-F
—

Use Case

Industrial raspberry ellagitannin ingredient production, premium functional berry extract manufacturing, beverage polyphenol scale-up. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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