Raspberry Ellagitannin IV-AG-B

Rubus idaeus

Raspberry

Tier IVRaspberry Ellagitannin

Target Trait

3.68×

fold-change

Sanguiin H-6/lambertianin C ellagitannin accumulation

Technical Specifications

SKU

SS-F-AST-786

Genomic Edits

GC Content

46.7%

Construct Length

4,326 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

368

Pathway Edits

UGGT overexpression (galloyl-UDP-glucosyltransferase) for galloyl-glucose platform establishment in Rubus
laccase-RAG amplification (Rubus-associated galloyl oxidase) for C-C oxidative coupling in sanguiin H-6 formation
BAHD-GalT galloyltransferase upregulation for sequential galloylation of glucose scaffold
PGT polygalloylglucoside transferase co-expression for lambertianin C extension
T-DNA right-border optimized integration cassette
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

UGGT/laccase-RAG coupled galloyl-glucose ester formation and oxidative C-C couplingBAHD-GalT sequential galloylation for sanguiin H-6 scaffold assemblyPGT polygalloylation for lambertianin C type ellagitannin polymerizationAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

368

IFI-F

—

Use Case

Industrial raspberry ellagitannin ingredient production, premium functional berry extract manufacturing, beverage polyphenol scale-up. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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