IFI-A · Galloylation Degree

Specimen SS-F-AST-754 // ASTRINGENCY · IFI-A

Galloylation Degree V-AG-B

Punica granatum

Pomegranate

Tier VGalloylation Degree

Target Trait

5.17×

fold-change

Procyanidin ester galloylation index

Technical Specifications

SKU

SS-F-AST-754

Genomic Edits

10

GC Content

47.5%

Construct Length

5,173 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

517

Pathway Edits

  • BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
  • GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
  • GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
  • GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
  • BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
517
IFI-F
—

Use Case

Flagship high-galloylation tannin ingredient production, pharmaceutical-grade gallotannin standardization, premium nutraceutical polyphenol formulation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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