Specimen SS-F-AST-752 // ASTRINGENCY · IFI-A
Galloylation Degree IV-IV-B
Punica granatum
Pomegranate
Target Trait
4.31×
fold-change
Procyanidin ester galloylation index
Technical Specifications
SKU
SS-F-AST-752
Genomic Edits
9
GC Content
46.2%
Construct Length
4,728 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
431
Pathway Edits
- BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
- GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
- GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
- GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
- BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Industrial high-galloylation tannin ingredient production, premium cosmetic polyphenol manufacturing, pharmaceutical nutraceutical formulation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
Already purchased?