IFI-A · Galloylation Degree

Specimen SS-F-AST-749 // ASTRINGENCY · IFI-A

Galloylation Degree IV-PR-A

Punica granatum

Pomegranate

Tier IVGalloylation Degree

Target Trait

3.75×

fold-change

Procyanidin ester galloylation index

Technical Specifications

SKU

SS-F-AST-749

Genomic Edits

8

GC Content

43.4%

Construct Length

4,297 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

375

Pathway Edits

  • BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
  • GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
  • GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
  • GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
  • BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
  • Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
375
IFI-F
—

Use Case

Industrial high-galloylation tannin ingredient production, premium cosmetic polyphenol manufacturing, pharmaceutical nutraceutical formulation.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Astringency · IFI-AAll Modalities