IFI-A · Galloylation Degree

Specimen SS-F-AST-748 // ASTRINGENCY · IFI-A

Galloylation Degree IV-BL-B

Punica granatum

Pomegranate

Tier IVGalloylation Degree

Target Trait

4.31×

fold-change

Procyanidin ester galloylation index

Technical Specifications

SKU

SS-F-AST-748

Genomic Edits

9

GC Content

47.0%

Construct Length

4,660 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

431

Pathway Edits

  • BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
  • GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
  • GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
  • GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
  • BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
  • Biolistic tungsten-particle coating for leaf/seed explant delivery
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
431
IFI-F
—

Use Case

Industrial high-galloylation tannin ingredient production, premium cosmetic polyphenol manufacturing, pharmaceutical nutraceutical formulation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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