IFI-A · Galloylation Degree
Specimen SS-F-AST-743 // ASTRINGENCY · IFI-A
Galloylation Degree III-IV-A
Punica granatum
Pomegranate
Tier IIIGalloylation Degree
Target Trait
3×
fold-change
Procyanidin ester galloylation index
Technical Specifications
SKU
SS-F-AST-743
Genomic Edits
7
GC Content
43.8%
Construct Length
3,984 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
300
Pathway Edits
- BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
- GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
- GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
- GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
- BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
- Cell-free wheat germ extract reconstitution system
Key Interventions
BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentSP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A300
IFI-F—
Use Case
Commercial high-galloylation tannin ingredient production, mid-range nutraceutical formulation, cosmetic polyphenol R&D.
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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