Galloylation Degree III-PR-B

Punica granatum

Pomegranate

Tier IIIGalloylation Degree

Target Trait

3.45×

fold-change

Procyanidin ester galloylation index

Technical Specifications

SKU

SS-F-AST-742

Genomic Edits

GC Content

47.6%

Construct Length

4,347 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

345

Pathway Edits

BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
Protoplast PEG-fusion transient and stable integration protocol
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

345

IFI-F

—

Use Case

Commercial high-galloylation tannin ingredient production, mid-range nutraceutical formulation, cosmetic polyphenol R&D. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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