Specimen SS-F-AST-728 // ASTRINGENCY · IFI-A
Galloylation Degree I-IV-B
Punica granatum
Pomegranate
Target Trait
1.72×
fold-change
Procyanidin ester galloylation index
Technical Specifications
SKU
SS-F-AST-728
Genomic Edits
5
GC Content
46.0%
Construct Length
3,107 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
172
Pathway Edits
- BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
- GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
- GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
- GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
- BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Galloylation degree research, pomegranate acyltransferase pathway studies, entry-level gallotannin precursor engineering. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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