IFI-A · Galloylation Degree
Specimen SS-F-AST-727 // ASTRINGENCY · IFI-A
Galloylation Degree I-IV-A
Punica granatum
Pomegranate
Tier IGalloylation Degree
Target Trait
1.5×
fold-change
Procyanidin ester galloylation index
Technical Specifications
SKU
SS-F-AST-727
Genomic Edits
4
GC Content
44.7%
Construct Length
2,910 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
150
Pathway Edits
- BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
- GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
- GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
- GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
- BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
- Cell-free wheat germ extract reconstitution system
Key Interventions
BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentSP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A150
IFI-F—
Use Case
Galloylation degree research, pomegranate acyltransferase pathway studies, entry-level gallotannin precursor engineering.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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