IFI-A · Galloylation Degree

Specimen SS-F-AST-725 // ASTRINGENCY · IFI-A

Galloylation Degree I-PR-A

Punica granatum

Pomegranate

Tier IGalloylation Degree

Target Trait

1.5×

fold-change

Procyanidin ester galloylation index

Technical Specifications

SKU

SS-F-AST-725

Genomic Edits

4

GC Content

43.9%

Construct Length

2,876 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

150

Pathway Edits

  • BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
  • GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
  • GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
  • GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
  • BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
  • Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
150
IFI-F
—

Use Case

Galloylation degree research, pomegranate acyltransferase pathway studies, entry-level gallotannin precursor engineering.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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