IFI-A · Galloylation Degree
Specimen SS-F-AST-721 // ASTRINGENCY · IFI-A
Galloylation Degree I-AG-A
Punica granatum
Pomegranate
Tier IGalloylation Degree
Target Trait
1.5×
fold-change
Procyanidin ester galloylation index
Technical Specifications
SKU
SS-F-AST-721
Genomic Edits
4
GC Content
44.2%
Construct Length
2,808 bp
Promoter System
Tissue-specific 2A11
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
Polyphenol-astringency axis
IFI Score
150
Pathway Edits
- BAHD-ATC overexpression (BAHD acyltransferase for galloylation) for ester-linked galloyl attachment
- GalT-I amplification (galloyl transferase I) for primary C-3 galloylation on procyanidin hydroxy groups
- GalT-II co-expression (galloyl transferase II) for secondary and tertiary galloylation positions
- GT-ester glucosyltransferase-esterase upregulation for galloyl-glucose ester substrate supply
- BAHD-ATC/GalT-I bicistron optimization for coupled galloyl activation and transfer
- T-DNA right-border optimized integration cassette
Key Interventions
BAHD-ATC/GalT-I/GalT-II galloylation cascade for graded ester galloylation degree controlGT-ester substrate supply for galloyl-glucose ester donor availabilityMulti-site galloylation architecture for astringency intensity and ellagitannin precursor enrichmentAgrobacterium co-cultivation with acetosyringone induction
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A150
IFI-F—
Use Case
Galloylation degree research, pomegranate acyltransferase pathway studies, entry-level gallotannin precursor engineering.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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