IFI-A · Robinetinidol Diversity

Specimen SS-F-AST-719 // ASTRINGENCY · IFI-A

Robinetinidol Diversity V-IV-A

Vaccinium corymbosum

Blueberry

Tier VRobinetinidol Diversity

Target Trait

2.8×

fold-change

Robinetinidol unit PA structural diversity index

Technical Specifications

SKU

SS-F-AST-719

Genomic Edits

7

GC Content

45.3%

Construct Length

4,038 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

280

Pathway Edits

  • CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
  • ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
  • LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
  • TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
  • Cell-free wheat germ extract reconstitution system

Key Interventions

CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversitySP6/T7 dual-polymerase cell-free transcription/translation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
280
IFI-F
—

Use Case

Flagship specialty PA diversity ingredient production, premium rare-unit polyphenol manufacturing, next-generation complex astringency engineering.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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