IFI-A · Robinetinidol Diversity
Specimen SS-F-AST-703 // ASTRINGENCY · IFI-A
Robinetinidol Diversity III-IV-A
Vaccinium corymbosum
Blueberry
Tier IIIRobinetinidol Diversity
Target Trait
1.95×
fold-change
Robinetinidol unit PA structural diversity index
Technical Specifications
SKU
SS-F-AST-703
Genomic Edits
5
GC Content
43.8%
Construct Length
3,144 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
195
Pathway Edits
- CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
- ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
- LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
- TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
- Cell-free wheat germ extract reconstitution system
Key Interventions
CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversitySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A195
IFI-F—
Use Case
Commercial specialty PA diversity ingredient production, mid-range complex astringency engineering.
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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