IFI-A · Robinetinidol Diversity

Specimen SS-F-AST-701 // ASTRINGENCY · IFI-A

Robinetinidol Diversity III-PR-A

Vaccinium corymbosum

Blueberry

Tier IIIRobinetinidol Diversity

Target Trait

1.95×

fold-change

Robinetinidol unit PA structural diversity index

Technical Specifications

SKU

SS-F-AST-701

Genomic Edits

5

GC Content

45.1%

Construct Length

3,110 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

195

Pathway Edits

  • CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
  • ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
  • LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
  • TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
  • Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversityPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
195
IFI-F
—

Use Case

Commercial specialty PA diversity ingredient production, mid-range complex astringency engineering.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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