Robinetinidol Diversity III-BL-B

Vaccinium corymbosum

Blueberry

Tier IIIRobinetinidol Diversity

Target Trait

2.24×

fold-change

Robinetinidol unit PA structural diversity index

Technical Specifications

SKU

SS-F-AST-700

Genomic Edits

GC Content

46.5%

Construct Length

3,673 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

224

Pathway Edits

CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
Biolistic tungsten-particle coating for leaf/seed explant delivery
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversityHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

224

IFI-F

—

Use Case

Commercial specialty PA diversity ingredient production, mid-range complex astringency engineering. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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