IFI-A · Robinetinidol Diversity

Specimen SS-F-AST-696 // ASTRINGENCY · IFI-A

Robinetinidol Diversity II-IV-B

Vaccinium corymbosum

Blueberry

Tier IIRobinetinidol Diversity

Target Trait

1.75×

fold-change

Robinetinidol unit PA structural diversity index

Technical Specifications

SKU

SS-F-AST-696

Genomic Edits

5

GC Content

47.3%

Construct Length

3,194 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

175

Pathway Edits

  • CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
  • ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
  • LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
  • TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversitySP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
175
IFI-F
—

Use Case

Craft rare-unit polyphenol ingredient sourcing, specialty PA diversity product development. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Astringency · IFI-AAll Modalities