IFI-A · Robinetinidol Diversity

Specimen SS-F-AST-694 // ASTRINGENCY · IFI-A

Robinetinidol Diversity II-PR-B

Vaccinium corymbosum

Blueberry

Tier IIRobinetinidol Diversity

Target Trait

1.75×

fold-change

Robinetinidol unit PA structural diversity index

Technical Specifications

SKU

SS-F-AST-694

Genomic Edits

5

GC Content

46.8%

Construct Length

3,160 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

175

Pathway Edits

  • CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
  • ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
  • LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
  • TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversityPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
175
IFI-F
—

Use Case

Craft rare-unit polyphenol ingredient sourcing, specialty PA diversity product development. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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