IFI-A · Robinetinidol Diversity

Specimen SS-F-AST-691 // ASTRINGENCY · IFI-A

Robinetinidol Diversity II-BL-A

Vaccinium corymbosum

Blueberry

Tier IIRobinetinidol Diversity

Target Trait

1.52×

fold-change

Robinetinidol unit PA structural diversity index

Technical Specifications

SKU

SS-F-AST-691

Genomic Edits

4

GC Content

43.5%

Construct Length

2,729 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

152

Pathway Edits

  • CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
  • ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
  • LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
  • TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
  • Biolistic tungsten-particle coating for leaf/seed explant delivery

Key Interventions

CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversityHigh-pressure helium particle acceleration at 1100 psi

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
152
IFI-F
—

Use Case

Craft rare-unit polyphenol ingredient sourcing, specialty PA diversity product development.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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