IFI-A · Robinetinidol Diversity
Specimen SS-F-AST-687 // ASTRINGENCY · IFI-A
Robinetinidol Diversity I-IV-A
Vaccinium corymbosum
Blueberry
Tier IRobinetinidol Diversity
Target Trait
1.1×
fold-change
Robinetinidol unit PA structural diversity index
Technical Specifications
SKU
SS-F-AST-687
Genomic Edits
3
GC Content
44.7%
Construct Length
2,450 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
110
Pathway Edits
- CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
- ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
- LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
- TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
- Cell-free wheat germ extract reconstitution system
Key Interventions
CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversitySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A110
IFI-F—
Use Case
Rare PA unit diversity research, blueberry polyphenol structural engineering, academic robinetinidol pathway studies.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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