Robinetinidol Diversity I-PR-B

Vaccinium corymbosum

Blueberry

Tier IRobinetinidol Diversity

Target Trait

1.26×

fold-change

Robinetinidol unit PA structural diversity index

Technical Specifications

SKU

SS-F-AST-686

Genomic Edits

GC Content

48.5%

Construct Length

2,813 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

126

Pathway Edits

CYP82D1 overexpression for 7,3,4,5-tetrahydroxyflavane ring modification and robinetinidol-type unit biosynthesis
ANR-alt alternative anthocyanidin reductase variant for regiochemically distinct substrate acceptance
LAR co-expression (leucoanthocyanidin reductase) for PA chain extension with robinetinidol units
TT12 vacuolar transporter upregulation for robinetinidol-containing PA sequestration
Protoplast PEG-fusion transient and stable integration protocol
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

CYP82D1 P450-mediated 7,3,4,5-tetrahydroxyflavane modification for robinetinidol unit biosynthesisANR-alt regiochemical flexibility for unusual leucoanthocyanidin substrate incorporationLAR/TT12 PA chain extension and vacuolar sequestration for robinetinidol diversityPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

126

IFI-F

—

Use Case

Rare PA unit diversity research, blueberry polyphenol structural engineering, academic robinetinidol pathway studies. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Astringency · IFI-A All Modalities