IFI-A · Leucoanthocyanidin Enhancement
Specimen SS-F-AST-679 // ASTRINGENCY · IFI-A
Leucoanthocyanidin Enhancement V-IV-A
Vaccinium corymbosum
Blueberry
Tier VLeucoanthocyanidin Enhancement
Target Trait
3×
fold-change
Leucodelphinidin accumulation index
Technical Specifications
SKU
SS-F-AST-679
Genomic Edits
7
GC Content
45.3%
Construct Length
3,958 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
300
Pathway Edits
- DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
- F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
- ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
- LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
- Cell-free wheat germ extract reconstitution system
Key Interventions
DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A300
IFI-F—
Use Case
Flagship leucoanthocyanidin ingredient production, premium prodelphinidin precursor manufacturing, pharmaceutical-grade leucodelphinidin standardization.
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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