Leucoanthocyanidin Enhancement V-AG-A

Vaccinium corymbosum

Blueberry

Tier VLeucoanthocyanidin Enhancement

Target Trait

3×

fold-change

Leucodelphinidin accumulation index

Technical Specifications

SKU

SS-F-AST-673

Genomic Edits

GC Content

45.9%

Construct Length

4,056 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

300

Pathway Edits

DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
T-DNA right-border optimized integration cassette

Key Interventions

DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplyAgrobacterium co-cultivation with acetosyringone induction

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

300

IFI-F

—

Use Case

Flagship leucoanthocyanidin ingredient production, premium prodelphinidin precursor manufacturing, pharmaceutical-grade leucodelphinidin standardization.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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