IFI-A · Leucoanthocyanidin Enhancement

Specimen SS-F-AST-666 // ASTRINGENCY · IFI-A

Leucoanthocyanidin Enhancement IV-AG-B

Vaccinium corymbosum

Blueberry

Tier IVLeucoanthocyanidin Enhancement

Target Trait

2.93×

fold-change

Leucodelphinidin accumulation index

Technical Specifications

SKU

SS-F-AST-666

Genomic Edits

7

GC Content

46.7%

Construct Length

3,906 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

293

Pathway Edits

  • DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
  • F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
  • ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
  • LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplyAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
293
IFI-F
—

Use Case

Industrial leucoanthocyanidin blueberry extract production, premium prodelphinidin precursor manufacturing, polyphenol ingredient scale-up. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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