IFI-A · Leucoanthocyanidin Enhancement
Specimen SS-F-AST-663 // ASTRINGENCY · IFI-A
Leucoanthocyanidin Enhancement III-IV-A
Vaccinium corymbosum
Blueberry
Tier IIILeucoanthocyanidin Enhancement
Target Trait
2.1×
fold-change
Leucodelphinidin accumulation index
Technical Specifications
SKU
SS-F-AST-663
Genomic Edits
5
GC Content
43.8%
Construct Length
3,264 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
210
Pathway Edits
- DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
- F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
- ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
- LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
- Cell-free wheat germ extract reconstitution system
Key Interventions
DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A210
IFI-F—
Use Case
Commercial leucoanthocyanidin ingredient production, mid-range prodelphinidin precursor R&D, blueberry polyphenol engineering.
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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