IFI-A · Leucoanthocyanidin Enhancement

Specimen SS-F-AST-656 // ASTRINGENCY · IFI-A

Leucoanthocyanidin Enhancement II-IV-B

Vaccinium corymbosum

Blueberry

Tier IILeucoanthocyanidin Enhancement

Target Trait

1.9×

fold-change

Leucodelphinidin accumulation index

Technical Specifications

SKU

SS-F-AST-656

Genomic Edits

5

GC Content

47.3%

Construct Length

3,114 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

190

Pathway Edits

  • DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
  • F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
  • ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
  • LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplySP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
190
IFI-F
—

Use Case

Craft leucoanthocyanidin ingredient development, specialty prodelphinidin precursor sourcing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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