IFI-A · Leucoanthocyanidin Enhancement
Specimen SS-F-AST-655 // ASTRINGENCY · IFI-A
Leucoanthocyanidin Enhancement II-IV-A
Vaccinium corymbosum
Blueberry
Tier IILeucoanthocyanidin Enhancement
Target Trait
1.65×
fold-change
Leucodelphinidin accumulation index
Technical Specifications
SKU
SS-F-AST-655
Genomic Edits
4
GC Content
45.4%
Construct Length
2,917 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
165
Pathway Edits
- DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
- F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
- ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
- LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
- Cell-free wheat germ extract reconstitution system
Key Interventions
DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A165
IFI-F—
Use Case
Craft leucoanthocyanidin ingredient development, specialty prodelphinidin precursor sourcing.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
Already purchased?