IFI-A · Leucoanthocyanidin Enhancement

Specimen SS-F-AST-649 // ASTRINGENCY · IFI-A

Leucoanthocyanidin Enhancement II-AG-A

Vaccinium corymbosum

Blueberry

Tier IILeucoanthocyanidin Enhancement

Target Trait

1.65×

fold-change

Leucodelphinidin accumulation index

Technical Specifications

SKU

SS-F-AST-649

Genomic Edits

4

GC Content

45.2%

Construct Length

2,815 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

165

Pathway Edits

  • DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
  • F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
  • ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
  • LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
  • T-DNA right-border optimized integration cassette

Key Interventions

DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplyAgrobacterium co-cultivation with acetosyringone induction

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
165
IFI-F
—

Use Case

Craft leucoanthocyanidin ingredient development, specialty prodelphinidin precursor sourcing.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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