Specimen SS-F-AST-648 // ASTRINGENCY · IFI-A
Leucoanthocyanidin Enhancement I-IV-B
Vaccinium corymbosum
Blueberry
Target Trait
1.38×
fold-change
Leucodelphinidin accumulation index
Technical Specifications
SKU
SS-F-AST-648
Genomic Edits
4
GC Content
46.0%
Construct Length
2,767 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
138
Pathway Edits
- DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
- F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
- ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
- LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Leucoanthocyanidin pathway research, blueberry flavan-3-ol intermediate studies, entry-level prodelphinidin precursor engineering. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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