IFI-A · Leucoanthocyanidin Enhancement

Specimen SS-F-AST-646 // ASTRINGENCY · IFI-A

Leucoanthocyanidin Enhancement I-PR-B

Vaccinium corymbosum

Blueberry

Tier ILeucoanthocyanidin Enhancement

Target Trait

1.38×

fold-change

Leucodelphinidin accumulation index

Technical Specifications

SKU

SS-F-AST-646

Genomic Edits

4

GC Content

48.5%

Construct Length

2,733 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

138

Pathway Edits

  • DFR-alt overexpression (3',5'-dihydroxyl-preferring dihydroflavonol 4-reductase allele)
  • F3-5H upregulation for B-ring trihydroxylation toward leucodelphinidin branch
  • ANS-KO (anthocyanidin synthase knockout) to block leucodelphinidin conversion to delphinidin
  • LAR-alt co-expression (alternative LAR allele with leucodelphinidin substrate preference)
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

DFR-alt allelic shift for leucodelphinidin reductase substrate preferenceANS-KO anthocyanin branch suppression to channel leucodelphinidin accumulationF3-5H trihydroxylation enhancement for leucodelphinidin precursor supplyPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
138
IFI-F
—

Use Case

Leucoanthocyanidin pathway research, blueberry flavan-3-ol intermediate studies, entry-level prodelphinidin precursor engineering. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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