Specimen SS-F-AST-640 // ASTRINGENCY · IFI-A
Phlobaphene Control V-IV-B
Sorghum bicolor
Sorghum
Target Trait
4.02×
fold-change
Flavan-4-ol/phlobaphene warm astringency index
Technical Specifications
SKU
SS-F-AST-640
Genomic Edits
8
GC Content
47.4%
Construct Length
4,275 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
402
Pathway Edits
- SG4 overexpression (sorghum flavan-4-ol synthase) for phlobaphene precursor production
- LAR co-expression (leucoanthocyanidin reductase) for PA branch balance modulation
- ANR upregulation (anthocyanidin reductase) to modulate phlobaphene:PA ratio
- F3H amplification (flavanone 3-hydroxylase) for dihydroflavonol substrate supply
- CHS enhancement (chalcone synthase) for naringenin-based substrate pool expansion
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Flagship sorghum warm-astringency ingredient production, premium functional beverage polyphenol formulation, industrial sorghum tannin extraction. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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