Phlobaphene Control IV-PR-B

Sorghum bicolor

Sorghum

Tier IVPhlobaphene Control

Target Trait

3.39×

fold-change

Flavan-4-ol/phlobaphene warm astringency index

Technical Specifications

SKU

SS-F-AST-630

Genomic Edits

GC Content

49.3%

Construct Length

3,894 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

339

Pathway Edits

SG4 overexpression (sorghum flavan-4-ol synthase) for phlobaphene precursor production
LAR co-expression (leucoanthocyanidin reductase) for PA branch balance modulation
ANR upregulation (anthocyanidin reductase) to modulate phlobaphene:PA ratio
F3H amplification (flavanone 3-hydroxylase) for dihydroflavonol substrate supply
CHS enhancement (chalcone synthase) for naringenin-based substrate pool expansion
Protoplast PEG-fusion transient and stable integration protocol
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

SG4-driven flavan-4-ol accumulation for sorghum-specific phlobaphene warm astringencyLAR/ANR competition modulation to balance phlobaphene and proanthocyanidin outputCHS/F3H substrate amplification for sustained flavan-4-ol biosynthesis capacityPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

339

IFI-F

—

Use Case

Industrial sorghum phlobaphene ingredient production, premium warm-astringency beverage formulation, functional polyphenol scale-up. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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