IFI-A · Phlobaphene Control

Specimen SS-F-AST-629 // ASTRINGENCY · IFI-A

Phlobaphene Control IV-PR-A

Sorghum bicolor

Sorghum

Tier IVPhlobaphene Control

Target Trait

2.95×

fold-change

Flavan-4-ol/phlobaphene warm astringency index

Technical Specifications

SKU

SS-F-AST-629

Genomic Edits

6

GC Content

43.4%

Construct Length

3,497 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

295

Pathway Edits

  • SG4 overexpression (sorghum flavan-4-ol synthase) for phlobaphene precursor production
  • LAR co-expression (leucoanthocyanidin reductase) for PA branch balance modulation
  • ANR upregulation (anthocyanidin reductase) to modulate phlobaphene:PA ratio
  • F3H amplification (flavanone 3-hydroxylase) for dihydroflavonol substrate supply
  • CHS enhancement (chalcone synthase) for naringenin-based substrate pool expansion
  • Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

SG4-driven flavan-4-ol accumulation for sorghum-specific phlobaphene warm astringencyLAR/ANR competition modulation to balance phlobaphene and proanthocyanidin outputCHS/F3H substrate amplification for sustained flavan-4-ol biosynthesis capacityPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
295
IFI-F
—

Use Case

Industrial sorghum phlobaphene ingredient production, premium warm-astringency beverage formulation, functional polyphenol scale-up.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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