Phlobaphene Control III-AG-B

Sorghum bicolor

Sorghum

Tier IIIPhlobaphene Control

Target Trait

2.76×

fold-change

Flavan-4-ol/phlobaphene warm astringency index

Technical Specifications

SKU

SS-F-AST-618

Genomic Edits

GC Content

48.2%

Construct Length

3,679 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

276

Pathway Edits

SG4 overexpression (sorghum flavan-4-ol synthase) for phlobaphene precursor production
LAR co-expression (leucoanthocyanidin reductase) for PA branch balance modulation
ANR upregulation (anthocyanidin reductase) to modulate phlobaphene:PA ratio
F3H amplification (flavanone 3-hydroxylase) for dihydroflavonol substrate supply
CHS enhancement (chalcone synthase) for naringenin-based substrate pool expansion
T-DNA right-border optimized integration cassette
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

SG4-driven flavan-4-ol accumulation for sorghum-specific phlobaphene warm astringencyLAR/ANR competition modulation to balance phlobaphene and proanthocyanidin outputCHS/F3H substrate amplification for sustained flavan-4-ol biosynthesis capacityAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

276

IFI-F

—

Use Case

Commercial sorghum tannin ingredient production, mid-range warm astringency engineering, functional beverage polyphenol R&D. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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