IFI-A · Phlobaphene Control
Specimen SS-F-AST-615 // ASTRINGENCY · IFI-A
Phlobaphene Control II-IV-A
Sorghum bicolor
Sorghum
Tier IIPhlobaphene Control
Target Trait
1.85×
fold-change
Flavan-4-ol/phlobaphene warm astringency index
Technical Specifications
SKU
SS-F-AST-615
Genomic Edits
4
GC Content
45.4%
Construct Length
2,837 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
185
Pathway Edits
- SG4 overexpression (sorghum flavan-4-ol synthase) for phlobaphene precursor production
- LAR co-expression (leucoanthocyanidin reductase) for PA branch balance modulation
- ANR upregulation (anthocyanidin reductase) to modulate phlobaphene:PA ratio
- F3H amplification (flavanone 3-hydroxylase) for dihydroflavonol substrate supply
- CHS enhancement (chalcone synthase) for naringenin-based substrate pool expansion
- Cell-free wheat germ extract reconstitution system
Key Interventions
SG4-driven flavan-4-ol accumulation for sorghum-specific phlobaphene warm astringencyLAR/ANR competition modulation to balance phlobaphene and proanthocyanidin outputCHS/F3H substrate amplification for sustained flavan-4-ol biosynthesis capacitySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A185
IFI-F—
Use Case
Craft sorghum warm-astringency beverage development, specialty flavan-4-ol ingredient sourcing.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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