IFI-A · Phlobaphene Control

Specimen SS-F-AST-606 // ASTRINGENCY · IFI-A

Phlobaphene Control I-PR-B

Sorghum bicolor

Sorghum

Tier IPhlobaphene Control

Target Trait

1.49×

fold-change

Flavan-4-ol/phlobaphene warm astringency index

Technical Specifications

SKU

SS-F-AST-606

Genomic Edits

4

GC Content

48.5%

Construct Length

2,853 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

149

Pathway Edits

  • SG4 overexpression (sorghum flavan-4-ol synthase) for phlobaphene precursor production
  • LAR co-expression (leucoanthocyanidin reductase) for PA branch balance modulation
  • ANR upregulation (anthocyanidin reductase) to modulate phlobaphene:PA ratio
  • F3H amplification (flavanone 3-hydroxylase) for dihydroflavonol substrate supply
  • CHS enhancement (chalcone synthase) for naringenin-based substrate pool expansion
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

SG4-driven flavan-4-ol accumulation for sorghum-specific phlobaphene warm astringencyLAR/ANR competition modulation to balance phlobaphene and proanthocyanidin outputCHS/F3H substrate amplification for sustained flavan-4-ol biosynthesis capacityPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
149
IFI-F
—

Use Case

Sorghum phlobaphene pathway research, entry-level flavan-4-ol engineering, academic sorghum tannin chemistry. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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