Salivary Protein Interaction IV-AG-B

Vitis vinifera

Grape

Tier IVSalivary Protein Interaction

Target Trait

3.89×

fold-change

Proline-rich protein binding tannin efficiency index

Technical Specifications

SKU

SS-F-AST-586

Genomic Edits

GC Content

46.7%

Construct Length

4,706 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

389

Pathway Edits

LAR balanced expression (leucoanthocyanidin reductase) for procyanidin catechin unit topology
ANR tuning (anthocyanidin reductase) for epicatechin extension unit frequency
TT12 vacuolar transport for procyanidin structure-preserving sequestration
TT19 glutathione S-transferase for PA sequestration efficiency optimization
LDOX co-expression (leucoanthocyanidin dioxygenase) for structural feature modulation of condensed tannin
T-DNA right-border optimized integration cassette
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Procyanidin chain topology optimization for proline-rich protein (PRP) binding kineticsLAR/ANR ratio control for PRP-binding structural features in condensed tanninLDOX/TT12 co-expression for vacuolar preservation of PRP-active tannin conformationAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

389

IFI-F

—

Use Case

Industrial precision astringency ingredient production, premium PRP-binding tannin manufacturing, astringency sensation engineering. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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