Salivary Protein Interaction III-IV-B

Vitis vinifera

Grape

Tier IIISalivary Protein Interaction

Target Trait

3.16×

fold-change

Proline-rich protein binding tannin efficiency index

Technical Specifications

SKU

SS-F-AST-584

Genomic Edits

GC Content

48.9%

Construct Length

4,261 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

316

Pathway Edits

LAR balanced expression (leucoanthocyanidin reductase) for procyanidin catechin unit topology
ANR tuning (anthocyanidin reductase) for epicatechin extension unit frequency
TT12 vacuolar transport for procyanidin structure-preserving sequestration
TT19 glutathione S-transferase for PA sequestration efficiency optimization
LDOX co-expression (leucoanthocyanidin dioxygenase) for structural feature modulation of condensed tannin
Cell-free wheat germ extract reconstitution system
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Procyanidin chain topology optimization for proline-rich protein (PRP) binding kineticsLAR/ANR ratio control for PRP-binding structural features in condensed tanninLDOX/TT12 co-expression for vacuolar preservation of PRP-active tannin conformationSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

316

IFI-F

—

Use Case

Commercial PRP-binding tannin ingredient production, mid-range astringency mechanism R&D, beverage mouthfeel optimization. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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