Salivary Protein Interaction II-BL-B

Vitis vinifera

Grape

Tier IISalivary Protein Interaction

Target Trait

2.45×

fold-change

Proline-rich protein binding tannin efficiency index

Technical Specifications

SKU

SS-F-AST-572

Genomic Edits

GC Content

49.1%

Construct Length

3,666 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

245

Pathway Edits

LAR balanced expression (leucoanthocyanidin reductase) for procyanidin catechin unit topology
ANR tuning (anthocyanidin reductase) for epicatechin extension unit frequency
TT12 vacuolar transport for procyanidin structure-preserving sequestration
TT19 glutathione S-transferase for PA sequestration efficiency optimization
LDOX co-expression (leucoanthocyanidin dioxygenase) for structural feature modulation of condensed tannin
Biolistic tungsten-particle coating for leaf/seed explant delivery
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Procyanidin chain topology optimization for proline-rich protein (PRP) binding kineticsLAR/ANR ratio control for PRP-binding structural features in condensed tanninLDOX/TT12 co-expression for vacuolar preservation of PRP-active tannin conformationHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

245

IFI-F

—

Use Case

Craft PRP-astringency engineering, specialty protein-binding tannin ingredient development. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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