IFI-A · Salivary Protein Interaction
Specimen SS-F-AST-567 // ASTRINGENCY · IFI-A
Salivary Protein Interaction I-IV-A
Vitis vinifera
Grape
Tier ISalivary Protein Interaction
Target Trait
1.5×
fold-change
Proline-rich protein binding tannin efficiency index
Technical Specifications
SKU
SS-F-AST-567
Genomic Edits
4
GC Content
44.7%
Construct Length
2,790 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
150
Pathway Edits
- LAR balanced expression (leucoanthocyanidin reductase) for procyanidin catechin unit topology
- ANR tuning (anthocyanidin reductase) for epicatechin extension unit frequency
- TT12 vacuolar transport for procyanidin structure-preserving sequestration
- TT19 glutathione S-transferase for PA sequestration efficiency optimization
- LDOX co-expression (leucoanthocyanidin dioxygenase) for structural feature modulation of condensed tannin
- Cell-free wheat germ extract reconstitution system
Key Interventions
Procyanidin chain topology optimization for proline-rich protein (PRP) binding kineticsLAR/ANR ratio control for PRP-binding structural features in condensed tanninLDOX/TT12 co-expression for vacuolar preservation of PRP-active tannin conformationSP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A150
IFI-F—
Use Case
Salivary interaction research, entry-level protein-tannin binding studies, academic mouthfeel mechanism investigation.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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