IFI-A · Salivary Protein Interaction

Specimen SS-F-AST-565 // ASTRINGENCY · IFI-A

Salivary Protein Interaction I-PR-A

Vitis vinifera

Grape

Tier ISalivary Protein Interaction

Target Trait

1.5×

fold-change

Proline-rich protein binding tannin efficiency index

Technical Specifications

SKU

SS-F-AST-565

Genomic Edits

4

GC Content

43.9%

Construct Length

2,756 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

150

Pathway Edits

  • LAR balanced expression (leucoanthocyanidin reductase) for procyanidin catechin unit topology
  • ANR tuning (anthocyanidin reductase) for epicatechin extension unit frequency
  • TT12 vacuolar transport for procyanidin structure-preserving sequestration
  • TT19 glutathione S-transferase for PA sequestration efficiency optimization
  • LDOX co-expression (leucoanthocyanidin dioxygenase) for structural feature modulation of condensed tannin
  • Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

Procyanidin chain topology optimization for proline-rich protein (PRP) binding kineticsLAR/ANR ratio control for PRP-binding structural features in condensed tanninLDOX/TT12 co-expression for vacuolar preservation of PRP-active tannin conformationPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
150
IFI-F
—

Use Case

Salivary interaction research, entry-level protein-tannin binding studies, academic mouthfeel mechanism investigation.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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