IFI-A · Pectin-Tannin Matrix

Specimen SS-F-AST-552 // ASTRINGENCY · IFI-A

Pectin-Tannin Matrix IV-IV-B

Vitis vinifera

Grape

Tier IVPectin-Tannin Matrix

Target Trait

3.43×

fold-change

Matrix-bound tannin mouthfeel sustained-release coefficient

Technical Specifications

SKU

SS-F-AST-552

Genomic Edits

8

GC Content

46.2%

Construct Length

4,348 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

343

Pathway Edits

  • PME overexpression (pectin methylesterase) for cell wall pectin demethylation and tannin association
  • PG1 upregulation (polygalacturonase 1) for pectin architecture remodeling
  • BAHD-GT glucosyltransferase amplification for polysaccharide-tannin linker formation
  • XTH co-expression (xyloglucan endotransglucosylase-hydrolase) for xyloglucan matrix optimization
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PME-mediated pectin demethylation for enhanced cell wall tannin associationPG1/BAHD-GT matrix remodeling for sustained mouthfeel tannin presentationXTH xyloglucan restructuring for optimized polysaccharide-tannin co-architectureSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
343
IFI-F
—

Use Case

Industrial sustained-release tannin ingredient production, premium texture-astringency engineering, beverage mouthfeel optimization. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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