Pectin-Tannin Matrix III-IV-B

Vitis vinifera

Grape

Tier IIIPectin-Tannin Matrix

Target Trait

2.82×

fold-change

Matrix-bound tannin mouthfeel sustained-release coefficient

Technical Specifications

SKU

SS-F-AST-544

Genomic Edits

GC Content

48.9%

Construct Length

4,001 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

282

Pathway Edits

PME overexpression (pectin methylesterase) for cell wall pectin demethylation and tannin association
PG1 upregulation (polygalacturonase 1) for pectin architecture remodeling
BAHD-GT glucosyltransferase amplification for polysaccharide-tannin linker formation
XTH co-expression (xyloglucan endotransglucosylase-hydrolase) for xyloglucan matrix optimization
Cell-free wheat germ extract reconstitution system
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PME-mediated pectin demethylation for enhanced cell wall tannin associationPG1/BAHD-GT matrix remodeling for sustained mouthfeel tannin presentationXTH xyloglucan restructuring for optimized polysaccharide-tannin co-architectureSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

282

IFI-F

—

Use Case

Commercial matrix-tannin ingredient production, mid-range mouthfeel engineering, functional beverage texture R&D. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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